AustralieFrV1, Main, Exploration, bibRecord, 007C55

Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice

Identifieur interne : 007C55 ( Main/Exploration ); précédent : 007C54; suivant : 007C56

Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice

Auteurs : Dagmar Wilhelm [Australie] ; Linda L. Washburn [États-Unis] ; Vy Truong [Australie] ; Marc Fellous [France] ; Eva M. Eicher [États-Unis] ; Peter Koopman [Australie]

Source :

Mechanisms of development [ 0925-4773 ] ; 2009.

RBID : PMC:2680453

Abstract

Ovotestis development in B6-XY^POS mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, Rspo1) development in B6-XY^POS ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not upregulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate Sox9; (2) challenge the established view that SOX9 is responsible for down-regulating Sry expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2680453

DOI: 10.1016/j.mod.2009.02.006
PubMed: 19269320
PubMed Central: 2680453

Affiliations:

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<front><div type="abstract" xml:lang="en"><p id="P2">Ovotestis development in B6-XY<sup>POS</sup>
 mice provides a rare opportunity to study the interaction of the testis- and ovary-determining pathways in the same tissue. We studied expression of several markers of mouse fetal testis (SRY, SOX9) or ovary (FOXL2, <italic>Rspo1</italic>
) development in B6-XY<sup>POS</sup>
 ovotestes by immunofluorescence, using normal testes and ovaries as controls. In ovotestes, SOX9 was expressed only in the central region where SRY is expressed earliest, resulting in testis cord formation. Surprisingly, FOXL2-expressing cells also were found in this region, but individual cells expressed either FOXL2 or SOX9, not both. At the poles, even though SOX9 was not upregulated, SRY expression was down-regulated normally as in XY testes, and FOXL2 was expressed from an early stage, demonstrating ovarian differentiation in these areas. Our data (1) show that SRY must act within a specific developmental window to activate <italic>Sox9</italic>
; (2) challenge the established view that SOX9 is responsible for down-regulating <italic>Sry</italic>
 expression; (3) disprove the concept that testicular and ovarian cells occupy discrete domains in ovotestes; and (4) suggest that FOXL2 is actively suppressed in Sertoli cell precursors by the action of SOX9. Together these findings provide important new insights into the molecular regulation of testis and ovary development.</p>
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Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice

Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice

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